ANTI-CMPK2 CLONE 1M13 ZOOMAB RABBIT MONOCLONAL (C15-1671-183)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1M13 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects mitochondrial UMP-CMP kinase 2 (CMPK2). It targets an epitope within 25 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human mitochondrial UMP-CMP kinase 2 (CMPK2).

Application

Quality Control Testing

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CMPK2 in THP-1 cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CMPK2 in K562 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CMPK2 in human small intestine tissue sections.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected CMPK2 in one million K562 and THP-1 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

UMP-CMP kinase 2, mitochondrial (UniProt: Q5EBM0; also known as EC:2.7.4.14, Cytidine/uridine monophosphate kinase 2, Nucleoside-diphosphate kinase) is encoded by the CMPK2 gene (Gene ID: 129607) in human. UMP-CMP kinase 2 is a mitochondrial enzyme that belongs to thymidine kinase family. It is synthesized with a transit peptide (aa 1-98), which is subsequently cleaved off to produce the mature form (aa 99-449). Its C-terminal domain contains all consensus motifs of a nucleoside monophosphate kinase. It participates in the synthesis of dUTP and dCTP in mitochondria. It phosphorylates dUMP, dCMP, CMP, and UMP with ATP as phosphate donor. However, its kinetic properties differ from cytosolic UMP-CMPK. It displays greatest efficacy to convert dUMP, followed by dCMP. CMP and UMP are relatively poor substrates. Its expression has been detected in chronic myelogenous leukemia K-562 and lymphoblastic leukemia MOLT-4 cells. Its high expression in leukemia cells, dominant expression in bone marrow, and tight correlation with macrophage activation and inflammatory response suggest that it may have other functions in addition to the supply of substrates for mtDNA synthesis. Four isoforms of UMP-CMP kinase 2 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Xu, Y., et al. (2008). J. Biol. Chem. 283(3); 1563-1571).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.