Anti-LILRB2 Antibody (C15-1317-324)

General description

Leukocyte immunoglobulin-like receptor subfamily B member 2 (UniProt Q8N423; also known as CD85 antigen-like family member D, CD85d, Ig-like transcript 4, ILT-4, Leukocyte immunoglobulin-like receptor 2, LIR-2, MIR-10, Monocyte/macrophage immunoglobulin-like receptor 10) is encoded by the LILRB2 (also known as CD85D, ILT4, LIR2, MIR10) gene (Entrez Gene ID 10288) in human. CD85d belongs to the B class of leukocyte immunoglobulin-like (LIR) receptors family, members of which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Cd85d is expressed on immune cells and negatively regulates immune response by engaging class I MHC molecules (HLA-A, HLA-B, HLA-C) on antigen-presenting cells.

Specificity

Reacts with all 4 known human CD85d isoforms.

Immunogen

Epitope: Extracellular domain

KLH-conjugated linear peptide corresponding to the extracellular domain of human LILRB2.

Application

Anti-LILRB2 Antibody is an antibody against LILRB2 for use in Western Blotting, Immunohistochemistry .

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LILRB2 in human ovarian cancer tumor cells, mouse cerebral cortex, human brain cancer tumor cells, mouse hippocampus, and Non-Hodgkin lymphoma cells.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected LILRB2 in 10 µg of Jurkat cell lysate.

Target description

~64/120 kDa observed. The calculated molecular weight of the four isoforms range from ~52 to ~65 kDa, a band with apparent molecular weight above ~110 kDa is often seen on blots as a result of glycosylation.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_005865. UniProt accession no.: Q8N423. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... LILRB2(10288). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.