Anti-LPA receptor 5 Antibody (GPR92) (C15-1317-959)

General description

Lysophosphatidic acid receptor 5 (LPA receptor 5), also known as GPR92, is a G12/13 and Gq coupled receptor that mediates the signaling of LPA. Ligand binding to LPA receptor 5 may increase intracellular levels of cAMP and calcium, resulting in the activation of a wide range of effector molecules. Recent studies have demonstrated that LPA receptor 5 is abundantly expressed in human mast cells, and mediates the release of the macrophage inflammatory protein-1β (MIP-1β), indicating that LPA receptor 5 may play a role in innate and adaptive immunity.

Specificity

This antibody recognizes the extracellular domain of LPA receptor 5.

Immunogen

Epitope: Extracellular domain

KLH-conjugated linear peptide corresponding to the extracellular domain of human LPA receptor 5.

Application

Detect the LPA receptor 5 protein using this Anti-LPA receptor 5 (GPR92) validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blot Analysis: A representative lot detected LPA receptor 5 in non-transfected CHEM-1 cells and CHEM-1 cells transfected with LPAR5(GPR92) (Catalogue. No. HTS209C - Transfected Cell Line).

Immunocychemistry Analysis: A 1:500 dilution from a representative lot detected LPAR5 in TF1 cells, non-transfected CHEM-1 cells and CHEM-1 cells transfected with LPAR5(GPR92) (Catalogue. No. HTS209C - Transfected Cell Line).

NOTE: The protein used to make the transfected CHEM1 cell line is full length LPAR5.

Quality

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected LPA receptor 5 on 10 µg of K562 cell lysate.

Target description

~37 kDa observed. The calculated molecular weight of this protein is 41 kDa, but can be observed at ~60 kDa due to high glycosylation.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
K562 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. technique(s): western blot: suitable. NCBI accession no.: NP_065133. UniProt accession no.: Q9H1C0. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... LPAR5(57121). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.