General description
The human gene NUP98 (Gene ID 4928; Also known as ADAR2, ADIR2, NUP96, NUP196) encodes the nuclear pore complex protein Nup98-Nup96 (UniProt P52948; also known as GLFG-repeat containing nucleoporin, Nup98-Nup96), which is posttranslationally autocleaved to form Nup98 and Nup96. Cleavage of the Nup98-96 linkage enhances the interaction between Nup98 and Nup96 and promotes localization of the two proteins to the nuclear pore complex (NPC). An alternative splice event generates a mRNA variant (isoform 3; also known as Nup98-specific 1), which expresses only the Nup98 portion of the Nup98-96 mRNA and allows the independent production of Nup98. Unlike Nup96, Nup98 is known to exist in both NPC-bound and non-NPC-associated pool, where it may participate in gene transcription regulations. Nup98 is reported to interact with histone-modifying enzymes CBP/p300 and histone deacetylases (HDACs) through its glycine-leucine-phenylalanine-glycine (GLFG) repeats. NUP98 gene is also involved in chromosomal translocations, resulting in Nup98 translocation fusions identified as potent transcription factors that induce acute myeloid leukemia (AML) with a particularly poor patient prognosis.
Specificity
Reacts with Nup98-Nup96 precursor, nucleoprotein Nup98, but not nucleoprotein Nup96.
Immunogen
Epitope: Near N-terminus
GST-tagged recombinant protein corresponding to human Nup98 near the N-terminus.
Application
Anti-Nup98 Antibody, clone 2H10 is an antibody against Nup98 for use in Western Blotting and Immunocytochemistry.
Immunocytochemistry Analysis: A representative lot detected Nup98 in Rat neurons.
Immunocytochemistry Analysis: A representative lot detected Nup98 nuclear localization in HeLa cells (Fukuhara, T., et al. (2005) Hybridoma (Larchmt). 24(5):244-247).
Immunocytochemistry Analysis: A representative lot detected nuclear localization Nup98 in NIH/3T3 cells (Oka, M., et al. (2010) Mol Biol Cell. 21(11):1885-1896).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Quality
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nup98 in 10 µg of HeLa nuclear extract.
Target description
~100 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2cκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 51162609
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABE1040
- Temperature Control Device:
- Yes
