Anti-RBPJ Antibody (C15-1317-139)

General description

Recombining binding protein suppressor of hairless (UniProt: Q06330; also known as CBF-1, Immunoglobulin kappa J region recombination signal binding protein 1, J kappa-recombination signal-binding protein, RBP-J, RBP-JK, RBP-J kappa, Renal carcinoma antigen NY-REN-30) is encoded by the RBPJ (also known as AOS3, CBF1, IGKJRB, IGKJRB1, KBF2, RBPJK, RBPSUH, SUH) gene (Gene ID: 3516) in human. RBP-J is a transcriptional regulator that plays a key role in Notch signaling. Acts as a transcriptional repressor when it is not associated with Notch proteins. It associates with several transcriptional co-repressors in the absence of Notch intracellular domain (NICD). Upon activation, following cleavage by gamma-secretase, NICD translocates to the nucleus and forms a transcriptional activation complex with RBPJ. This complex in turn recruits other co-activators and complex components, leading to regulated target gene expression. Genome-wide ChIP-seq profiling reveals both overlapping and non-overlapping chromatin-binding sites occupied by RBPJ and Notch1. Murine RBPJ-only sites are shown to be highly enriched for imputed REST (a DNA-binding transcriptional repressor) sites, whereas human RPBJ-only sites lack REST motifs and are more enriched for imputed CREB sites. Dysregulated RBPJ-dependent gene expression is seen in B-cell tumors associated with Epstein-Barr virus (EBV) and a majority of T-lymphoblastic leukemia/lymphoma (TLL) tumors having somatic Notch1 gain-of-function mutations.

Specificity

This rabbit polyclonal antibody recognizes Recombining binding protein suppressor of hairless (RBP-J) in human and murine cells. It targets and epitope within the first 48 amino acids in the N-terminal region.

Immunogen

Epitope: N-terminus

GST-tagged recombinant fragment corresponding to the first 48 amino acids of human Recombining binding protein suppressor of hairless (RBP-J).

Application

Anti-RBPJ, Cat. No. ABE384, is a highly specific rabbit polyclonal antibody that targets Recombining binding protein suppressor of hairless (RBP-J) and has been tested in ChIP-seq, Chromatin Immunoprecipitation (ChIP), and Western Blotting.

Chromatin Immunoprecipitation Analysis: A representative lot detected RBP-J in human T-cell acute lymphoblastic leukemia (T-ALL) cell line CUTLL1 (Wang, H., et. al. (2011). Proc Natl Acad Sci USA. 108(36):14908-13).

Western Blotting Analysis A 1:2,000 dilution from a representative lot detected RBP-J in GM12878 lymphoblasts (Courtesy of Dr Zhao Bo).

ChIP-seq Analysis: A representative lot detected RBP-J in ChIP-seq applications (Wang, H., et. al. (2011). Proc Natl Acad Sci USA. 108(36):14908-13).

Western Blotting Analysis: A representative lot detected RBPJ in T-cell acute lymphoblastic leukemia (T-ALL) cell lines of both human (KOPT-K1 , HPB-ALL, and CUTLL1) and murine (T6E and G4A2) origins) (Wang, H., et. al. (2011). Proc Natl Acad Sci USA. 108(36):14908-13).

Chip-seq Analysis: A representative lot detected RBPJ in GM12878 cells (Courtesy of Dr Zhao Bo).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RBPJ in 10 µg of HEK293 cell lysate.

Target description

~65 kDa observed; 55.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit polyclonal in serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human. species reactivity (predicted by homology): primate (based on 100% sequence homology). technique(s): ChIP: suitable (ChIP-seq), western blot: suitable. NCBI accession no.: NP_005340. UniProt accession no.: Q06330. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... RBPJ(3516). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.