Anti-RBPMS Antibody (C15-1317-342)

General description

RNA-binding protein with multiple splicing (UniProt Q93062; also known as Heart and RRM expressed sequence, Hermes, RBP-MS) is encoded by the RBPMS (also known as HERMES) gene (Gene ID 11030) in human. RBPMS is a member of the RRM (RNA recognition motif) family of RNA-binding proteins. Transcriptome-wide study using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in HEK293 cells reveals both exonic mature and intronic pre-mRNA-binding sites, in agreement with the nuclear and cytoplasmic localization of the RRM family proteins. RBPMS is found to be exclusively expressed in retinal ganglion cells (RGCs) and displaced RGCs (dRGCs). RBPMS expression can therefore be utitized as a RGC marker, and the RBPMS gene promoter could be utilized for driving RGC-specific transgene expression.

Specificity

Expected to react with all five spliced isoforms (UniProt Q93062-1 through Q93062-5).

Immunogen

Epitope: N-terminal region.

KLH-conjugated linear peptide corresponding to a sequence from the N-terminal region of human RBPMS.

Application

Anti-RBPMS Antibody is an antibody against RBPMS for use in Western Blotting, Immunofluorescence.

Research Category
Neuroscience

Research Sub Category
Sensory & PNS

Western Blotting Analysis: A representative lot detected endogenous RBPMS in mouse and rat retina extracts, as well as exogenously expressed human RBPMS in transfected HEK293T cells (Rodriguez, A.R., et al. (2014). J. Comp. Neurol. 522(6):1411-1443).
Immunofluorescence Analysis: A representative lot detected RBPMS immunoreactivity primarily associated with cell bodies located in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry using whole-mounted retinas (Rodriguez, A.R., et al. (2014). J. Comp. Neurol. 522(6):1411-1443).
Immunofluorescence Analysis: An 1:5,000 dilution of a representative lot detected RBPMS immunoreactivity among cells in the ganglion cell layer (GCL) of paraformaldehyde-fixed mouse retinas by fluorescent immunohistochemistry (Courtesy of Dr. Nicholas Brecha, David Geffen School of Medicine at UCLA).

Quality

Evaluated by Western Blotting in mouse embryonic stem cell (mESC) lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RBPMS in 10 µg of mouse embryonic stem cell (mESC) lysate.

Target description

~25 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal antibody in PBS with 1% BSA and 0.09 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse, rat. technique(s): immunofluorescence: suitable, western blot: suitable. NCBI accession no.: NP_001008710. UniProt accession no.: Q93062. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... RBPMS(11030). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.