General description
The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Specificity
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody is suitable for ELISA, immunoblotting and dot blotting assays.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
biological source: mouse. Quality Level: 200. conjugate: alkaline phosphatase conjugate. antibody form: purified from hybridoma cell culture. antibody product type: primary antibodies. clone: HIS-1, monoclonal. form: buffered aqueous glycerol solution. technique(s): western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein. isotype: IgG2a. shipped in: wet ice. storage temp.: 2-8°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US).- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- A5588-.5ML
