General description
Recombinant, human monocyte chemotactic protein-1 expressed in E. coli. A potent chemoattractant for human monocytes and mouse peritoneal macrophages. Its effects are proposed to be mediated by P2-integrins.
Biochem/physiol Actions
ED₅₀ = 5-20 ng/ml as measured by its monocyte chemotactic activity
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg MCP-1.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.
Other Notes
Hosang, K., et al. 1994. Biochem. Biophys. Res. Commun.199, 962.
Luini, W., et al. 1994. Cytokine 6, 28.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Quality Level: 100. Assay: ≥. 97% (SDS-PAGE). form: lyophilized. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. impurities: ≤. 1 . EU/μ. g Endotoxins (EU/μ. g MCP-1). shipped in: ambient. storage temp.: −. 70°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41106202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 475901-10UG
