General description
Note: 1 mU = 1 milliunit.
Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides.
Recombinant, Chryseobacterium meningosepticum N-Glycosidase F expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of N-glycans from high mannose, hybrid and complex glycopeptides, and from glycoproteins generating asparagine-linked oligosaccharides. Supplied with a series of buffers.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will completely catalyze the release of N-linked oligosaccharides from 1 µmol denatured ribonuclease in 1 min at 37°C, pH 7.5.
Physical form
Enzyme supplied in 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Fan, J.-Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Altmann, F., et al. 1995. Glycoconj. J. 12, 84.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 362300-1SET
