Nissl Staining Solution

Aladdin's Nissl Staining Solution can be used to stain Nissl bodies in plasma of neuron cells preserved in paraffin or frozen sections. The Nissl Staining Solution contains Cresyl violet which can bind to RNA on the rough endoplasmic reticulum and DNA in nucleus and stain cell body in mottled blue-purple.Nissl Stain named after Franz Nissl, a German psychiatrist and neuropathologist, stains Nissl bodies in blue-purple, and is often used by neurobiologist to display the basic neural structure of brain or spinal cord. A large number of Nissl bodies indicates that nerve cells are active in protein synthesis, while the number of Nissl bodies will decrease or even disappear in damaged nerve cells.This product is sufficient for staining 200 samples.Precautions：Special Note: Nissl stain has a strong staining capability and is difficult to remove. Please take effective measures to avoid contact with skin or clothing.When there is a large number of samples, staining racks or tanks produced by can be used for easier operation.For first-time users of this product, we recommend performing a preliminary test with 1-2 samples.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear lab coat and disposable gloves during the operation.Instructions for Use：1. Sample preparationa. For paraffin sections:Dewax paraffin sections thrice in xylene for 5-10 minutes each. Note: Insufficient dewaxing will cause uneven sample staining.Anhydrous ethanol for 5 minutes;90% ethanol for 2 minutes;70% ethanol for 2 minutes;Distilled water for 2 minutes;Proceed to step 2.b. For frozen sections:Fix frozen sections with 4% paraformaldehyde for more than 10 minutes;Wash sections with distilled water for 2 minutes;Replace with new distilled water and wash for another 2 minutes;Proceed to step 2.c. For cultured cells:Fix cells with 4% paraformaldehyde for more than 10 minutes;Wash with distilled water for 2 minutes;Replace with fresh distilled water and wash for another 2 minutes;Proceed to step 2.2. Nissl staininga. Stain samples with Nissl Staining Solution for 5-10 minutes (The duration of time can be adjusted appropriately).b. Rinse with distilled water twice for a few seconds each time.c. Wash with 95% ethanol for 5 seconds.d. Wash samples with 70% ethanol twice, examine cells directly or proceed to step 3a or step 3b.Note: To counterstain sample that has been immunohistochemistry stained, the above procedures can be performed directly after the completion of immunohistochemistry staining.3. Dehydration, clearing, mounting or fluorescence staininga. Dehydration, clearing, and mountingDehydrate samples with 95% ethanol for 2 minutes;Replace with fresh 95% ethanol and dehydrate for another 2 minutes;Replace with xylene and clear for 5 minutes;Replace with fresh xylene and clear for another 5 minutes;Use neutral gum or other mounting medium to mount the slide;Examine samples by light microscopy. Cells should be stained in mottled blue-purple.b. Fluorescence stainingAfter staining with Nissl, immunofluorescence staining or other fluorescence staining, such as Hoechst staing, can be performed as follows:Following step 2d, wash samples with 70% ethanol twice, 2 minutes each.Immerse samples for 5 minutes in PBS, saline, TBS or TBST solution used for immunostaining or fluorescence staining.Proceed to immunofluorescence staining or other fluorescence staining following appropriate protocols.