Potato Dextrose Agar with 3% Agar is for cultivation of yeasts and moulds from dairy and other food products. Pack size is 2.5KG

Potato Dextrose Agar is recommended by APHA (1) and F.D.A (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Incorporation of 3% agar enhances sporulation in the medium. Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify. Directions: Suspend 54 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.