Cell Line Origin
Rabbit epidermis, cottontail
Cell Line Description
From minced ear epidermis of adult male cottontail rabbit with papillomatosis. Cells can be cultured continuously at pH 7.4 and 37°C but may also be adequately maintained at 25°C. Developed for studies of epidermis specific virus replication. Also know as Sf1Ep (NBL-11).
Application
Virus replication studies (may be useful for viruses producing lesions in skin)
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan. Maximum of 80 passages expected. Cells become spindle shaped when confluent.
Other Notes
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biological source: rabbit skin. growth mode: Adherent. karyotype: 2n : 42. morphology: Epithelial. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 42121606
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 90092702-1VL
