1,4-Dithiothreitol (DTT) (C007B-130600)

OverviewReducing agentApplicationUse 1,4-Dithiothreitol primarily to protect free SH-groups from oxidation. Use it routinely in all work with enzymes and proteins during enzyme measurement and the characterization of proteins.• As one of the reduction and alkylation reactants of the main allergen αs1-casein in milk.• As a component of sperm membrane removal and reactivation medium.•Because mercaptans can effectively protect the active sites of biocatalysts and maintain the stability of enzymes.• As a reducing agent to test the substitution of N-alkaline maleic substitution and the reaction of excess radicals.Before SDS-PAGE, DTT can effectively reduce protein disulfide bonds in the sample buffer. Both the irritation and toxicity of DTT are reduced by 2-mercaptoethanol. Usually, the required concentration of DTT (100mM) is used. It uses one-seventh of the concentration of 2-cholesterol-based ethanol (5% v/v, 700 mM).Benefits1.Rely on the higher stability of DTT in comparison to 2-mercaptoethanolin aqueous solution2.Experience more convenience and a less disagreeable odor3.Benefit from the reduced tendency to oxidize in air.SpecificationAppearance: White to yellowish crystallizateDTT (with Ellman's reagent): ≥97%Thin layer chromatography: Chromatographically homogeneous, corresponds to referenceStability: At +2 to +8°C within specification range for 24 months. Store dry. Protect from light. Specification: ≥97% MDL Number: MFCD00004877 Molecular Formula: C4H10O2S2 Molecular Weight: 154.25 EC Number: MFCD00004877 PubChem CID: 446094 Isomeric SMILES: C([C@H]([C@@H](CS)O)O)S Beilstein Registry Number: 1719760 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/D265376-SCI_b82ded6df0c2f175e2afe85a259eaee8.pdf