Cell Line Origin
Breast cancer, morphology, adhesion properties
Cell Line Description
1-7HB2 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 identified by screening for branching morphology. The MTSV1-7 is a clonal non-tumourigenic cell line developed by immortalizing luminal epithelial cells cultured from milk using the SV40 T antigen. (See also MTSV1-7 CE1 Sigma catalog number 10081202.). Used for in vitro study of branching morphogenesis of human mammary epithelial cells and the role of integrins in mammary morphogenesis.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17
Culture Medium
DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
- UPC:
- 41102421
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10081201-1VL