Application
100 mM IPTG Solution - Novagen has been used to:
- induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells
- induce expression in calcium-independent sortase from BL21(DE3) cells
- turn on bacterial protein production of the protein A/G-MNase
Biochem/physiol Actions
Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.
Warning
Toxicity: Carcinogenic / Teratogenic (D)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 41116124
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 70527-3