10X PCR Buffer without MgCl₂ (C09-1020-566)

Description10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl2, each dNTP at 200 μM, primers defining an approximately 500 base pair region of λ DNA at 1.0μM each, λ DNA template at 1ng/100μL, and Taq DNA polymerase at 2.5 units/100μL. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments and 72 °C to extend the DNA segments. Following electrophoresis of the reaction products in 1.5% agarose gel, a single band of approximately 500 base pairs was visualized for PCRs containing 1-1.5mM MgCl2.10X PCR Buffer without MgCl2has been used as a component of polymerase chain reaction (PCR) amplification mixture for the mycotoxigenic mould DNA, parasite DNA andFusarium oxysporumDNA amplification.10× PCR Buffer without MgCl2has been used with Sigma′s PCR enzymes. Specifications and Purity: Optimized for routine PCR without MgCl₂.