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11-4

11-4

Catalog No.
C15-1296-700
Manufacturer No.
11033102-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI
Price: $1,630.29
List Price: $1,811.43

Cell Line Origin HeLa, genetic modification, firefly luciferase reporter gene, HSV-thymidine kinase promoter Cell Line Description 11-4 is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the

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Cell Line Origin

HeLa, genetic modification, firefly luciferase reporter gene, HSV-thymidine kinase promoter

Cell Line Description

11-4 is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the HSV-thymidine kinase promoter. 11-4 can be used to evaluate the non-specific transcriptional activity of chemicals.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

UPC:
41106600
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
11033102-1VL


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