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1184 human lung fibroblast (c15-1311-543)

1184 HUMAN LUNG FIBROBLAST (C15-1311-543)

Sigma Aldrich

Catalog No.
C15-1311-543
Manufacturer No.
90011883-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI
Price: $1,325.68
List Price: $2,039.50

Cell Line Origin Human lung fibroblast Cell Line Description A thioguanine resistant derivative of MRC-5 CV1, selected with 5μg/ml 6-thioguanine. Application Genetic studies DNA Profile STR-PCR Data: Amelogenin: X,Y CSF1PO: 11,12 D13S317:

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Cell Line Origin

Human lung fibroblast

Cell Line Description

A thioguanine resistant derivative of MRC-5 CV1, selected with 5μg/ml 6-thioguanine.

Application

Genetic studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: human lung. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
12352206
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
90011883-1VL

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