Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
Not specified
Serological Profile
HLA A: 3 32
HLA C: 2 7
HLA B: 7 61
HLA DR: 2 13
HLA DQ: 0 1
Culture Medium
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
biological source: human blood. ethnicity: Caucasian. gender: female. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice.- UPC:
- 41115709
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94031643-1VL
