1x McCoy 5A Medium, Iwakata & Grace Modification with L-Glutamine, 500mL

1× McCoy 5A Medium, Iwakata & Grace Modification with L-Glutamine is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and transformed cell types, and the most demanding primary and continuous cell lines. McCoy’s media were originally formulated for the growth and support of human lymphocytes. The 5A modification originated as a reformulation of McCoy’s by Hsu and Kellogg. It was then further modified by Iwakata and Grace featuring increased levels of i-inositol and glucose. This final modification produced a media identical to RPMI 1629. McCoy’s 5A media support the indefinite proliferation of Walker 256 carcinoma cells. Originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used in the culture of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.