The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.Application:2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product. It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.
Specification: Liquid Substrate System
MDL Number: MFCD00214141
- UPC:
- 51101687
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.62 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- A755502-100ml
- Product Size:
- 100ml
