2-(2-Pyridyl)ethyl Silica Gel (C15-1305-487)

General description

Retention Mechanism: Anion-exchange
Sample Matrix Compatibility: Organic or aqueous Solutions

• Weak anion exchanger ideal for extracting strong basic compounds that remain charged at all pH levels
• Unlike conventional weak anion exchange SPE phases such as -NH₂ (aminopropyl) that have a pKa of 9-10, a pH ≤ 7 is required to protonate or ionize the stationary phase to facilitate analyte retention. Elution is typically done by increasing the pH to 11 resulting in neutralization of the SPE phase.
• 2-(2-pyridyl)-ethyl silica gel has a pKa of ~6. Therefore, analyte elution is feasible at a pH ≥ 7. This characteristic is important for extracting analytes that are not stable (e.g. hydrolyzes) at high pHs typically required for elution when using traditional weak anion exchangers.
• Ideal for extracting acyl-coenzyme A esters from tissue.
• For more information, please see: Minkler, P.E., Kerner, J., Ingalls, S.T., Hoppel, C.L., Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue, Analytical Biochemistry 376 (2008) 275–276

composition: bed wt, 100 . mg . Quality Level: 100. packaging: pk of 108. technique(s): solid phase extraction (SPE): suitable. volume: 1 . mL . matrix active group: WAX phase. application(s): food and beverages. separation technique: ion exchange. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H315 - H319 - H335. Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.