2-Iminobiotin (C09-0919-127)

2-Iminobiotin is a cyclic guanidino analog of biotin that has high affinity for avidin at high pH (>9) and low affinity at low pH (<6). The proteins that are iminobiotinylated are selectively contained within the avidin column at pH 9 to 11 and may be eluted at pH 4 or by the addition of biotin. This technique may be very useful in the study of cell surface proteins by labeling and selectively recovering the components that are periodate-sensitive from intact human erythrocyte surface.2-Iminobiotin reversibly inhibits NOS (nitric oxide synthases) via its guanidino group. It inhibits NO biosynthesis and may be important to understand the binding-site interactions for this important class of enzymes. NOS oxidizes the guanidino-nitrogen of L-arginine to produce nitric oxide and L-citrullineA reversible inhibitor of mouse NOS2 (iNOS) and rat NOS1 (nNOS). Specifications and Purity: 98%. MDL Number: MFCD00066150. Molecular Formula: C10H17N3O2S. Molecular Weight: 243.33. PubChem CID: 128878. Isomeric SMILES: C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)O)N=C(N2)N. Beilstein Registry Number: 18952. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/I113701-SCI_0767c333bbde8f829dea0ebd689a9631.pdf.