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2-phosphoglycerate assay kit

2-Phosphoglycerate Assay Kit

Aladdin

Catalog No.
C09-1020-591
Manufacturer No.
P486339-1kit
Manufacturer Name
Aladdin Scientific
Quantity
1
Unit of Measure
EA
Price: $1,709.88
List Price: $1,899.87

Description2-Phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway, a critical mechanism of ATP generation. It is converted to phosphoenol pyruvate (PEP), catalyzed by the enzyme enolase. The consumption of glucose and the

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Description2-Phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway, a critical mechanism of ATP generation. It is converted to phosphoenol pyruvate (PEP), catalyzed by the enzyme enolase. The consumption of glucose and the rate of glycolysis is upregulated in metastatic cancers, a phenomenon known as the Warburg effect. Measurement of intracellular levels of 2PG is a tool for analyzing the glycolytic pathway in cancer cells.Suitability: Suitable for the detection of 2-Phosphoglycerate in tissue and cell culture samples.Principle: The 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit is a simple assay that measures 2PG in various samples (ranging from 2-10 nmole/well for the colorimetric assay and from 50-250 pmole/well for the fluorometric assay). 2PG concentration is determined by converting the 2PG to PEP and then to pyruvate. The pyruvate is oxidized, resulting in a colorimetric (570 nm) or fluorometric (λex= 535/ λem= 587 nm) product proportional to the amount of 2PG present.Preparation instructionsPrincipleThe 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit is a simple assay that measures 2PG in various samples (ranging from 2-10 nmole/well for the colorimetric assay and from 50-250 pmole/well for the fluorometric assay). 2PG concentration is. Specification: Sufficient for 100 Colorimetric or Fluorometric tests Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P486339-SCI_fdf22fa65ab4679394e6f668754708a0.pdf
UPC:
12161503
Condition:
New
Availability:
8-12 weeks
Weight:
3.53 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
P486339-1kit
Product Size:
1kit


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