2 × SYBR Green qPCR Kit (C09-1038-162)

Product IntroductionComposition50T×50μL reaction system 200T×50μL reaction system 2×SYBR Green qPCR Mix1.25 mL1.25 mL×4Product parameterEx(nm)：497Em(nm)：525Storage: Store at -20 ℃ in the dark for 6 months. Dissolve and mix thoroughly before use. It can be stored at 4℃ for short-term use to avoid repeated freezing and thawing.Product descriptionThis product is a special reagent for Real Time PCR using SYBR Green I chimeric fluorescence method. Reagents such as DNA polymerase, dNTP, special stabilizers, optimized reaction buffer, BSA and SYBR Green I have been premixed into a 2×Premix Type reagent suitable for Real Time PCR reaction detection. It has high sensitivity, strong specificity, Features such as good stability. When using, only need to add template, primer and water, a good standard curve can be obtained in a wide quantitative area, and the target gene can be accurately and quantitatively detected, with good repeatability and high reliability.Precautions 1. This product does not contain the reference dye ROX. Customers can decide whether to add ROX reference dye according to the qPCR instrument technical guidance to eliminate the signal background and correct the fluorescence signal error generated between the wells.2. This product contains SYBR Green I. It is easy to decompose under strong light and reduce sensitivity. Avoid long-term strong light exposure to this product.3. It is recommended to prepare the PCR reaction solution on ice and put it into the PCR instrument for amplification. It can improve amplification specificity and reduce background.4. This product contains 4 mM MgCl2 (the final concentration of the reaction system is 2 mM Mg2+), and 25mM MgCl2 can be used to optimize the Mg2+ concentration.Suggested PCR conditions (take a 50 μL reaction system as an example, please prepare the reaction solution on ice)ComponentsVolumeFinal Concentration2×SYBR Green qPCR Mix25μL1×DNA Template2μLas requiredForward Primer (10μM)1μL0.2 μM eachReverse Primer (10μM)1μL0.2 μM eachddH2O to final volume50μLNot applicablePCR cycle94℃     2～3 min                     94℃     10～20 sec55～65℃   10～20 sec    35～45 cycles72℃     20～60 sec72℃    5～10 min. Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/S272945-SCI_16e02094c12e16601cf2951ef1b5a04f.pdf