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2X Laemmli Sample Buffer

Aladdin

Catalog No.
C09-1092-276
Manufacturer No.
X378399-30ml
Manufacturer Name
Aladdin Scientific
Quantity
1
Unit of Measure
EA
Price: $23.80
List Price: $26.44

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces

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Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.2X Laemmli Sample Bufferis for SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer补充资料This is classified as a Dangerous Good for transport and may be subject to additional shipping charges. Specification: 2X Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/X378399-SCI_89ce32cb336a56118f8459a6f356b16e.pdf
UPC:
12161703
Condition:
New
Availability:
8-12 weeks
Weight:
1.92 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
X378399-30ml
Product Size:
30ml
Hazard Statement Codes:
H302:H311:H318
Precautionary Statement Codes:
P264:P270:P280

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