General description
This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.
Application
3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:
- to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectant
- to measure the levels of α-hemolysin toxin
- in imaging bound IgGs
3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
- UPC:
- 41116158
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- T4444-4X100ML
- Product Size:
- 4/EA
- CAS:
- 54827-17-7
