Application
3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.
Biochem/physiol Actions
DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.
Packaging
Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.
Caution
Injecting a compatible solvent permits preparation of any desired strength solution without exposure.
Reconstitution
Dissolving the contents in 100 mL of solvent yields a 0.1% solution.
Legal Information
Isopac is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 51413107
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- D9015-100MG