3-HBDH GRADE II (C15-1295-692)

General description

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.

Application

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.

Biochem/physiol Actions

3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-­hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.

Quality

Contaminants: <0.1% LDH, <5% MDH

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Stabilizers: NADH, Ca²⁺

Other Notes

For life science research only. Not for use in diagnostic procedures.