3D Collagen Culture Kit

General description

Cell migration is a fundamental function of normal cellular processes, including cell proliferation, and migration. Cell behavior in vitro is typically examined in a 2D environment; however, in the body, cells exist in a three-dimensional extracellular matrix environment rich in type I collagen. In fact, cells cultured in 3D matrices better reflect in vivo cell physiology when compared to traditional 2D systems.

CHEMICON (EMD Millipore) has developed a three-dimensional cell culture system that closely simulates the collagen-rich extracellular matrix of normal tissue. This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.

As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.

For Research Use Only; Not for use in diagnostic procedures

Application

Research Category
Cell Structure

This 3D system provides a simple and rapid method to analyze cell angiogenesis, migration, apoptosis, proliferation and tissue formation in a 3D-collagen matrix. Cells suspended in this 3D system are easily visualized by phase contrast or fluorescence light microscopy. Cells can be directly fixed and stained within the matrix and treated with antibodies for visualization of specific intra- and extracellular proteins. Importantly, cells suspended in our 3D system can also be treated with various reagents allowing for extensive screening of biological responses to growth factors and chemical agents.

As an additional benefit to investigators, sterile and viable cells may be removed from the 3D Cell Culture System for further experimentation, including FACS or biochemical analysis.

Components

Collagen Solution - (Part No. 90135) One bottle - 10 mL

5X RPMI Medium- (Part No. 90134) One bottle - 2.5 mL

5X M199 Medium- (Part No. 90133) One bottle - 2.5 mL

5X DMEM Medium- (Part No. 90137) One bottle - 2.5 mL

5X PBS with Phenol Red- (Part No. 90136) One bottle - 2.5 mL

Neutralization Solution- (Part No. 90138) One vial - 0.5 mL

Physical form

Collagen solution is supplied in 6mM acetate buffer at ~ pH7.0.

Storage and Stability

Store kit materials at 4°C up to their expiration date.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.