Application
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) has been used:
- as a protease inhibitor in plasma samples to prevent acylated ghrelin (AG) degradation
- as a snake venom serine proteinase (SVSP) inhibitor in SVSP inhibition or AEBSF assay to test the involvement of SVSPs on the fibrinogen-clotting activity
- as a component in NP-40 lysis buffer to resuspend cell pellets for preparing cell lysates
Recommended concentrations for use are 0.1-1 mM.
Biochem/physiol Actions
AEBSF can inhibit acetylcholinesterase (AChE).
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an irreversible serine protease inhibitor. Inhibition constants are similar to those of phenylmethanesulfonyl fluoride (PMSF) and diisopropylfluorophosphate (DFP). AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein, and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water, and improved stability in aqueous solutions. AEBSF can be used in cell culture in concentrations of up to 0.25 mM.
- UPC:
- 51144011
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- A8456-500MG
- CAS:
- 30827-99-7
