Cell Line Origin
Mouse Sp2/0 Ag-14 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against a 144kDa protein present predominantly on the apical, but also on the basolateral surfaces of Madin-Darby canine kidney cells of the MDCK cell line.
Immunogen
Basolateral membrane fraction from dog intestine
Application
Study of cell surface polarity development
Culture Medium
EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: Sp2/0 Ag-14. Reactivity: Anti-Madin-Darby canine kidney cells. technique(s): cell culture | mammalian: suitable. isotype: IgG1, kappa. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 12352207
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 93062218-1VL
- Temperature Control Device:
- Yes