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4-methylumbelliferyl n-acetyl-beta-d-glucosaminide (c15-1891-397)

4-METHYLUMBELLIFERYL-N-ACETYL-B-D- GLUCOSAMINIDE CRYSTALLINE (C005B-454588)

Catalog No.
C005B-454588
Mfr. No.
M2133-100MG
Mfr. Name
Sigma-Aldrich
Qty/UOM
100
UOM
MG
Price: $389.75
List Price: $433.05

General description 4-Methylumbelliferyl N -acetyl-β:- D -glucosaminide (4-MUF-NAG) contains chitin monomer, N -acetyl- D -glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety. Application

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General description

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) contains chitin monomer, N-acetyl-D-glucosamine (NAG) and a 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF) moiety.

Application

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a substrate for assaying

  • β-N-acetylglucosaminidase (NAGase) from soil samples
  • chitinase from Fusarium oxysporum using fluorimetric assay
  • β-N-acetylhexosaminidases from Arabidopsis thaliana roots and leaves samples

Biochem/physiol Actions

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic fluorogenic substrate for N-acetyl-β-D-glucosaminidase (NAGase). The enzyme functionality is assessed by its ability to hydrolyze the N-acetyl-D-glucosamine (NAG) residue in 4-MUF-NAG. 4-MUF-NAG is also a substrate for Chitinase and N-acetylhexosaminidase.

Substrates

Fluorogenic substrate for β-N-acetylhexosaminidase

UPC:
51303200
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
M2133-100MG
CAS:
37067-30-4

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