4-Nitrophenyl butyrate (C09-0976-585)

4-Nitrophenyl butyrate is a nitro-substituted phenolic ester compound. 4-Nitrophenyl butyrate is water-soluble and is tolerated as a substrate by lipase enzymes, making it a useful probe for lipase activity. Hydrolysis of 4-Nitrophenyl butyrate by the enzyme produces p-nitrophenoxide which can be monitored by UV-Vis spectrophotometry at 400 nm. This approach has specifically been used to assess the activities of both cholesterol esterase and lipoprotein lipase and 4-Nitrophenyl butyrate has also been employed as a probe for screening the lipolytic activity of novel microorganism strains.A probe for lipase activity.Application4-nitrophenyl butyrate has been used: as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity as a substrate to determine the effect of buffers and solvents on human carboxylesterase. Specification: ≥98% MDL Number: MFCD00047734 Molecular Formula: C10H11NO4 Molecular Weight: 209.2 PubChem CID: 75834 Isomeric SMILES: CCCC(=O)OC1=CC=C(C=C1)[N+](=O)[O-] Beilstein Registry Number: 1879729 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/N138584-SCI_fc044d61801b3c597ba92a2ccf7a0793.pdf