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4-NITROPHENYL N-ACETYL-BETA-D-GLUCOSAMINIDE (C005B-443732)

Catalog No.
C005B-443732
Mfr. No.
N9376-100MG
Mfr. Name
Sigma-Aldrich
Qty/UOM
100
UOM
MG
Price: $177.87
List Price: $197.63

Application N -acetyl-β:- D -glucosaminidase (NAG) has been used: as a substrate to assay N -acetyl-β:- D -glucosaminidase (NAG) as a substrate to study chitinase ChiB1 activity to assess degranulation using rat basophilic leukemia

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Application

N-acetyl-β-D-glucosaminidase (NAG) has been used:

  • as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)
  • as a substrate to study chitinase ChiB1 activity
  • to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells

Biochem/physiol Actions

4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.

Substrates

Chromogenic substrate for N-acetyl-glucosaminidase.

UPC:
12352200
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
N9376-100MG
CAS:
3459-18-5

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