P-NITROPHENYL PHOSPHATE DITRIS (C005B-444385)

Catalog No.: C005B-444385
Mfr. No.: N3254-5G
Mfr. Name: Sigma-Aldrich
Qty/UOM: 5
UOM: GM

Price: $233.04

List Price: $258.93

Application 4-Nitrophenyl phosphate di( tris ) salt has been used: as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates to determine phosphatase activity using native polyacrylamide gel electrophoresis

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Description
Specifications

Application

4-Nitrophenyl phosphate di(tris) salt has been used:

as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

UPC:: 51171622
Condition:: New
Availability:: 3-5 Days
Weight:: 1.00 Ounces
HazmatClass:: No
MPN:: N3254-5G
CAS:: 68189-42-4

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