General description
5-Bromo-4-chloro-3-indolyl β -
Application
5-Bromo-4-chloro-3-indolyl β-
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Biochem/physiol Actions
X-gal is routinely used to detect transgene activity in situ.
Quantity
Contains 5 mg substrate per tablet
Reconstitution
A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.
- UPC:
- 51112605
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- B6024-50TAB