5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (C09-0745-072)

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indoly?l phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.Histochemical substrate for alkaline phosphatase. Specifications and Purity: ≥99%(HPLC). MDL Number: MFCD00040636. Molecular Formula: C15H15BrClN2O4P. Molecular Weight: 433.62. EC Number: 229-506-1. PubChem CID: 81059. Isomeric SMILES: CC1=CC=C(C=C1)N.C1=CC(=C(C2=C1NC=C2OP(=O)(O)O)Cl)Br. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/B105547-SCI_8406eb0ca31dada49e0406cd9d4a7c04.pdf