5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt (C09-0780-141)

5-Bromo-6-chloro-3-indoxyl phosphate p-toluidine salt, also known as Magenta-phos p-toluidine salt, is a histochemical substance used for visualizing areas of alkaline phosphatase activity. Hydrolysis of the phosphate group on 5-Bromo-6-chloro-3-indoxyl phosphate by alkaline phosphatase generates an insoluble indigo compound. The indoxyl species resulting from phosphate hydrolysis of related compounds can be reacted with tetrazolium salts, reducing the tetrazolium to a colorful insoluble formazan species. A solubilization technique involving reduction of the insoluble hydrolytic product of the closely related 5-Bromo-4-chloro-3-indoxyl phosphate permitted the use of this substrate in quantitative ELISA experiments. 5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt is a substrate of TNAP. Specifications and Purity: ≥98%. Molecular Formula: C15H15BrClN2O4P. Molecular Weight: 433.62. PubChem CID: 192667. Isomeric SMILES: CC1=CC=C(C=C1)N.C1=C2C(=CC(=C1Br)Cl)NC=C2OP(=O)(O)O. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/B334413-SCI_7b241b87248203312732962a667e0658.pdf