Application
5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.
Molecular Weight: 1057.82. Empirical Formula: C42H54N5O21P3. Assay: >. 95% (HPLC). Quality Level: 200. form: solution. reaction suitability: reaction type: click chemistry. solubility: Tris buffer: 100 . mM (pH 7.5). shipped in: wet ice. storage temp.: −. 20°C. SMILES string: O: C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O): O): O): O)O2)O)C: C1/C: C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4: CC: CC: C4C#CC5: CC: CC: C53): O): O): O): O. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.- UPC:
- 41115710
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 809705-50UL
