Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
The hybridoma cell line 55-6 produces antibodies reacting with the Human Immunodeficiency Virus I (HIV-1) envelope protein, gp120. Spleen cells from BALB/c mice that had been sequentially immunised with recombinant gp120(rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB,SF2 and Z6) have been fused with Sp2/0-Ag14 myeloma cells. The antibody binds rgp120 from the III B isolate and reacts weakly with gp120 from the MN strain, both shown by ELISA. No cross blocking of any other antibodies in the developed panel was detected, thus indicating that 55-6 binds to a unique site on gp120IIIB. In peptide recognition assays it was shown to bind a synthetic peptide 105-117 (in the CI region) of gp120.
Immunogen
rgp 120
Application
gp120 assays
Culture Medium
RPMI 1640 + 2mM glutamine + HT + 10% FBS
Subculture Routine
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2;37ºC
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: Mouse BALB/c spleen. Myeloma: Sp2/0-Ag14. Reactivity: Anti-HIV-1 gp120. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97040308-1VL
