Cell Line Origin
Rat medullary thyroid carcinoma
Cell Line Description
Derived from the non-clonal line rMTC 6-23 originally established from a transplantable WAG/RIJ rat medullary thyroid carcinoma. The cell line synthesises and secretes both neurotensin and calcitonin. Its clonal derivation demonstrates that a population derived from a single type of C-cell has the capability of producing both peptides. Secretion of these peptides has been shown to be stimulated by calcium, KC1, glucagon and catecholamines.
Application
Hormone studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells need to be grown on flasks coated with matrigel or any other attachment factor
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. For cell attachment culture vessels need to be coated with collagen type IV e.g. Sigma product C-5533. Soak in a sol
Other Notes
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biological source: rat thyroid. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Neurotensin and calcitonin. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51180000
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 87042206-1VL
- Temperature Control Device:
- Yes
