Cell Line Origin
Human anti-hepatitis C virus IgG1 secreting B cell
Cell Line Description
Derived from a patient with chronic Hepatitis C (HCV type 1). Antibody specific to non structural protein 4a (NS4a) of Hepatitis C virus. Fine specificity mapped to amino acid residues 1697-1708 of HCV deduced amino acid sequence. The cells should be handled under laboratory containment level 2 conditions.
Application
Used in HCV typing studies.
Culture Medium
RPMI 1640 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 20u/ml IL-6 + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x10,000 cells/ml; using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Disclaimer
This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.
biological source: human blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblast. products: Immunoglobulin G (IgG1) (kappa) specific for Hepatitis C virus NS4 region. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41181510
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94111519-1VL