7-Deaza-2'-deoxyguanosine 5'-triphosphate lithium salt

Application

7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt has been used as a lysis agent to evaluate its efficiency on direct cell lysis, RNA stability, and compatibility with downstream reverse transcription quantitative real-time PCR during the analyzes of samples with small numbers of cells.

Biochem/physiol Actions

7-Deaza-2′-deoxyguanosine 5′-triphosphate (7-deaza-dGTP) competes with natural substrate dGTP and blocks the telomerase activity. This nucleotide once incorporated into the telomere alters the secondary structure of the telomere making it difficult for telomerase to identify it for further telomere synthesis. This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA polymerases. 7-deaza-dGTP in combination with dimethyl sulfoxide (DMSO) and betaine enhances the polymerase chain reaction (PCR) amplification of GC-rich DNA sequences.