Cell Line Origin
Mouse Sp2/0 x Mouse spleen
Cell Line Description
8D12 and 8A12 used in a sandwich ELISA giving a sensitive assay for biological samples. The antibody is directed against the guinea pig major basic protein found in guinea pig eosinophils. It can be paired in a sandwich ELISA with 8A12.
Immunogen
Guinea pig major basic protein
Application
Bioassay
Culture Medium
IDMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 1-2x100,000 cells/ml; 5% CO2; 37°C. Saturation density is reached at 1x1000,000 cells/ml.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: Mouse spleen. Myeloma: Sp2/0. Reactivity: Anti-Guinea Pig Major Basic Protein. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 12352207
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94091320-1VL