A-KG Dehydrogenase Activity Assay Kit

Descriptionα-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is a highly regulated Krebs cycle enzyme, forms the E1 subunit of the mitochondrial 2-oxoglutarate dehydrogenase complex. It catalyzes the conversion of α-ketoglutarate to succinyl-CoA and produces NADH directly providing electrons for the respiratory chain. Decreased α-KGDH complex activity has been associated with oxidative stress and neurodegenerative disorders such as Alzheimer′s and Parkinson′s diseases.α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of α-ketoglutarate dehydrogenase in samples.Suitability: Suitable for the detection of α-ketoglutarate dehydrogenase activity in samples such as tissue, cells, and purified mitochondria.Principle: The α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a simple procedure for measuring α-ketoglutarate dehydrogenase activity in a variety of tissues, cells, and isolated mitochondria. α-KGDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of α-ketoglutarate dehydrogenase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.5 at 37 °C. Specifications and Purity: Sufficient for 100 Colorimetric tests. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/A486239-SCI_7e818c0062171447d899f399de428210.pdf