Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against a 188kDa polypeptide present on the basolateral plasma membrane of Madin-Darby canine kidney (MDCK) cells. This polypeptide is the same as, or closely related to, the cell adhesion molecule uvomorulin.
Immunogen
Madin-Darby canine kidney (MDCK) cells
Application
Study of epithelial occluding barriers
Culture Medium
EMEM(EBSS) + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-Madin-Darby canine kidney cell E-cadherin (uvomorulin). technique(s): cell culture | mammalian: suitable. isotype: IgG1, kappa. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51112522
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93062220-1VL