Cell Line Origin
Human caucasian metastatic melanoma
Cell Line Description
This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human skin (lymph node metastasis). growth mode: Adherent. karyotype: Not specified. morphology: Epithelial. products: Not specified. receptors: Nerve growth factor (NGF), Laminin. technique(s): cell culture | mammalian: suitable. relevant disease(s): metastasis. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51112513
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 91100402-1VL