Cell Line Origin
Human malignant melanoma IL-1 insensitive
Cell Line Description
The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.
Application
IL-1 resistance studies, receptor studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% FBS
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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biological source: human skin. description: Human malignant melanoma IL-1 insensitive. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41105328
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97011320-1VL