Cell Line Origin
Human malignant melanoma IL-1 sensitive
Cell Line Description
The cell line A375-C6 has been shown to be sensitive to the IL-1 mediated anti-proliferative effect. It has been derived from the parental line A375 (Sigma Catalogue number. 88113005) by limiting dilution together with its twin clone A375-C5 (Sigma Catalogue number. 97011320) which is IL-1 unresponsive. A375-C6 could be useful for a growth inhibition assay for IL-1. The cells have been eradicated from mycoplasma at ECACC.
Application
IL-1 growth inhibition assay, IL-1 receptor analysis
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 1-3 x 10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: human skin. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial-like. products: Not specified. receptors: IL-1 receptor. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41116126
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97011321-1VL